Semiquantitative RT-PCR measurement of gene expression in rat tissues including a correction for varying cell size and number

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Semiquantitative RT-PCR measurement of gene expression in rat tissues including a correction for varying cell size and number

BACKGROUND Current methodology of gene expression analysis limits the possibilities of comparison between cells/tissues of organs in which cell size and/or number changes as a consequence of the study (e.g. starvation). A method relating the abundance of specific mRNA copies per cell may allow direct comparison or different organs and/or changing physiological conditions. METHODS With a numbe...

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Investigation of beta defensin gene expression in the ocular anterior segment by semiquantitative RT-PCR.

AIM To determine if beta defensins are expressed in the anterior segment of the eye and to determine the temporal pattern of expression using a real time semiquantitative reverse transcription polymerase chain reaction (RT-PCR). METHODS Ocular tissue (corneal epithelium, conjunctiva, iris, and lens capsule) was collected from 23 patients undergoing surgery. Serial corneal or conjunctival impr...

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Investigation of â defensin gene expression in the ocular anterior segment by semiquantitative RT-PCR

Aim—To determine if â defensins are expressed in the anterior segment of the eye and to determine the temporal pattern of expression using a real time semiquantitative reverse transcription polymerase chain reaction (RT-PCR). Methods—Ocular tissue (corneal epithelium, conjunctiva, iris, and lens capsule) was collected from 23 patients undergoing surgery. Serial corneal or conjunctival impressio...

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RT-PCR and in situ hybridization analysis of apolipoprotein H expression in rat normal tissues.

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ژورنال

عنوان ژورنال: Nutrition & Metabolism

سال: 2007

ISSN: 1743-7075

DOI: 10.1186/1743-7075-4-26